19 February 2010 Pump-probe optical coherence microscopy
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High-resolution optical molecular imaging has become a vital tool for understanding and measuring physiologically important biometrics on the cellular and subcellular level. In spite of significant recent advances in microscopy, molecular imaging of most endogenous biomolecular species remains elusive. Directly imaging endogenous biomolecules without the aid of exogenous tags is highly desirable. We developed pump-probe optical coherence microscopy (PPOCM) based on our previous success in integrating pump-probe absorption spectroscopy with optical coherence tomography. A fixed human skin tissue with melanoma was imaged by the PPOCM system. The preliminary results show that PPOCM can provide better can clear contrast between normal tissue and melanoma than OCM. This system also can be used to image other chromophores.
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Qiujie Wan, Qiujie Wan, Brian E. Applegate, Brian E. Applegate, } "Pump-probe optical coherence microscopy", Proc. SPIE 7554, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIV, 75541Z (19 February 2010); doi: 10.1117/12.843310; https://doi.org/10.1117/12.843310

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