Lab-on-a-chip or Micro total analysis systems (μTAS) technologies offer a lot of potential applications for biosensing
and biomedical detections. This paper presents the design, fabrication and characterization of a fully integrated siliconpolymer
based biophotonic Micro-Total Analysis System for the real-time detection of enzymes and antigens. This
device uses optical detection methods i.e, optical absorption, Laser induced fluorescence and evanescence measurement
technique to detect the presence, concentration and the activity of biomolecules. The main components of the proposed
system are microfluidic unit and micromechanical fluid actuation system, integrated with the optical detection systems.
An Echelle grating based Spectrometer-on-Chip on Silica-on-Silicon (SOS) is integrated with the opto-microfluidic
assembly for fluorescence detection. On-Chip fabrication and integration of valveless micropump has been carried out in
order to facilitate the transportation of fluid within the system. The important advantages of the proposed μTAS are
functional independence of each module of the system, simultaneous multi-analyte detection, rapid, precise and
discriminating results, low background/high signal-to-noise ratio, lack of moving parts, robust, portability, and
feasibility of bulk fabrication.