24 February 2010 Dissociation constant measurement using combination tapered fiber optic biosensor (CTFOB) dip-probes
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The document reports a novel method of measuring dissociation constant (kD) of antibody-antigen interaction using evanescent wave based combination tapered fiber-optic biosensor (CTFOB) dip-probes. The method was demonstrated by measuring the dissociation constant of human Interleukin-6 (IL-6) and anti-IL-6 interaction. Sandwich immunoassay was used to generate fluorescence signal proportional to antigen-antibody conjugate pairs. A portable CCD based spectrometer setup was used to record spectral profile of the fluorescence signal. The measured value of dissociation constant kD for IL-6 and capture anti-IL-6 (clone MQ2-13A5) antibodies at room temperature is588 ±19 pM .
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Chun-Wei Wang, Chun-Wei Wang, Rakesh Kapoor, Rakesh Kapoor, "Dissociation constant measurement using combination tapered fiber optic biosensor (CTFOB) dip-probes", Proc. SPIE 7559, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications X, 75590A (24 February 2010); doi: 10.1117/12.843720; https://doi.org/10.1117/12.843720

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