11 February 2010 Optical fiber bundle coupling errors in Raman spectra: correction via data processing
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Light can be coupled into imaging spectrographs through the use of fiber-optic bundles. Ideally, the collected spectra from adjacent optical fibers should be resolved and independent. However, this assumption breaks down if a partial overlap of adjacent fibers on the detector CCD results from either diffraction or uncorrected monochromatic aberrations. In addition, spectral mixing can be caused by optical cross-talk among tightly packed fibers, particularly if the fiber-optic buffer has been removed in order to use the CCD area more efficiently and increase the linear fiber packing density. These coupling effects can become sources of systematic error, especially when fiber bundles are used for imaging or when branches of a multi-leg fiber array are interrogating different samples. Coupling errors can mix spectra from different spatial regions of the sample. In this paper, the mixing in fiber bundles will be assessed, and mathematical methods to resolve overlapped signals and correct for signal mixing will be discussed. Using data processing to correct for optical coupling errors, instead of physically changing the spacing between fibers in the bundle, allows for the efficient use of the limited spectrograph detector area by having a larger number of fibers.
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Kathryn A. Dooley, Kathryn A. Dooley, Francis W. L. Esmonde-White, Francis W. L. Esmonde-White, Michael D. Morris, Michael D. Morris, } "Optical fiber bundle coupling errors in Raman spectra: correction via data processing", Proc. SPIE 7560, Biomedical Vibrational Spectroscopy IV: Advances in Research and Industry, 75600O (11 February 2010); doi: 10.1117/12.842418; https://doi.org/10.1117/12.842418

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