Application of NIR fluorescent markers to quantify expression level of HER2 receptors in carcinomas in vivo

Victor Chernomordik; Moinuddin Hassan; Sang Bong Lee; Rafal Zielinski; Jacek Capala; Amir Gandjbakhche

doi:10.1117/12.855652

23 February 2010 Application of NIR fluorescent markers to quantify expression level of HER2 receptors in carcinomas in vivo

Victor Chernomordik, Moinuddin Hassan, Sang Bong Lee, Rafal Zielinski, Jacek Capala, Amir Gandjbakhche

Author Affiliations +

Proceedings Volume 7561, Optical Biopsy VII; 756118 (2010) https://doi.org/10.1117/12.855652
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

HER2 overexpression has been associated with a poor prognosis and resistance to therapy in breast cancer patients. However, quantitative estimates of this important characteristic have been limited to ex vivo ELISA essays of tissue biopsies and/or PET. We develop a novel approach in optical imaging, involving specific probes, not interfering with the binding of the therapeutic agents, thus, excluding competition between therapy and imaging. Affibody-based molecular probes seem to be ideal for in vivo analysis of HER2 receptors using near-infrared optical imaging. Fluorescence intensity distributions, originating from specific markers in the tumor area, can reveal the corresponding fluorophore concentration. We use temporal changes of the signal from a contrast agent, conjugated with HER2-specific Affibody as a signature to monitor in vivo the receptors status in mice with different HER2 over-expressed tumor models. Kinetic model, incorporating saturation of the bound ligands in the tumor area due to HER2 receptor concentration, is suggested to analyze relationship between tumor cell characteristics, i.e., HER2 overexpression, obtained by traditional ("golden standard") ex vivo methods (ELISA), and parameters, estimated from the series of images in vivo. Observed correlation between these parameters and HER2 overexpression substantiates application of our approach to quantify HER2 concentration in vivo.

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Victor Chernomordik, Moinuddin Hassan, Sang Bong Lee, Rafal Zielinski, Jacek Capala, and Amir Gandjbakhche "Application of NIR fluorescent markers to quantify expression level of HER2 receptors in carcinomas in vivo", Proc. SPIE 7561, Optical Biopsy VII, 756118 (23 February 2010); https://doi.org/10.1117/12.855652

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KEYWORDS

Tumors

Receptors

In vivo imaging

Luminescence

Optical imaging

Cancer

Near infrared

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