22 February 2010 Molecular delivery into live cells using gold nanoparticle coated substrates fabricated by pulsed laser annealing
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Abstract
A massively-parallel molecular delivery system for mammalian cells is demonstrated by pulsed-laser irradiation of a gold-nanoparticles-coated substrate situated below a cell monolayer. This system is capable of high throughput and spatially-targeted delivery into desired areas of a cell culture by designing the laser irradiation pattern. Large area, rapid fabrication of the gold-nanoparticle-coated substrate is achieved by pulsed laser annealing of a continuous gold thin film. Randomly distributed gold nanoparticles and periodic gold nanoparticle arrays were obtained by pulsed laser flood exposure and by polymer mold guided laser annealing respectively. Optical image patterned molecular delivery into adherent cells were demonstrated in HeLa and HEK 293T cells. Delivery efficiencies of fluorescent dye, calcein, were as high as >90% with high cell viability (>90%) in HEK 293T using this device.
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Ting-Hsiang Wu, Fan Xiao, Michael A. Teitell, Pei-Yu Chiou, "Molecular delivery into live cells using gold nanoparticle coated substrates fabricated by pulsed laser annealing", Proc. SPIE 7562, Optical Interactions with Tissues and Cells XXI, 756203 (22 February 2010); doi: 10.1117/12.840732; https://doi.org/10.1117/12.840732
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