22 February 2010 Spatiofrequency filters for imaging fluorescence in scattering media
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Abstract
Researchers have been using simple optics to image optically induced fluorescence in tissues. We now apply the Angular Domain Imaging technique using a Spatiofrequency filter which accepts only photons within a small deviation angle from its original trajectory to image a fluorescing medium beneath a scattering layer. A Rhodamine 6 G dye fluorescing layer or fluorescence slides, under an Intralipid scattering medium was used. By applying ADI with acceptance angle of 0.17°, the structures are distinguishable at low scattering depth depending of the emission wavelength of the fluorescence source. It was established previously that as the acceptance angle increases, the amount of scattered light/noise in the images increases, however, the resolution also deteriorates. Simulations using a Monte-Carlo program are done for both angular filters, Spatiofrequency filter and Linear Collimating Array. Due to the additional positional filtration on top of the angular filtration with Linear Collimating Array, collimators with aspect ratio as low as 10:1 can improve the quality of the fluorescence images significantly in both contrast ratio and resolution.
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Polly Tsui, Polly Tsui, Glenn H. Chapman, Glenn H. Chapman, Rongen L. K. Cheng, Rongen L. K. Cheng, Gary Chiang, Gary Chiang, Nick Pfeiffer, Nick Pfeiffer, Bozena Kaminska, Bozena Kaminska, } "Spatiofrequency filters for imaging fluorescence in scattering media", Proc. SPIE 7562, Optical Interactions with Tissues and Cells XXI, 75620B (22 February 2010); doi: 10.1117/12.842998; https://doi.org/10.1117/12.842998
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