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11 February 2010 The effect of temperature of fluorescence: an animal study
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The effect of temperature on the fluorescence of enucleated porcine eyes and rat skin was studied. The fluorescence peak intensity was found to decrease as the tissue temperature increased. A dual-excitation, fiber-based system was used to collect fluorescence and diffuse-reflectance spectra from the samples. A thermal camera was used to determine the temperature of the tissue at the time of fluorescence measurement. The samples were mounted in a saline bath and measurements were made as the tissue temperature was increased from -20°C to 70°C. Results indicate that temperature affects several fluorescence spectra characteristics. The peak height decreased as temperature increased. At temperatures above 60°C, the peak position shifted to lower wavelengths. Heating and cooling experiments of the rat skin demonstrate the recovery of the loss in fluorescence. The diffuse reflectance spectra indicated a change in optical properties past 60°C, but prior to the denaturation temperature for collagen at 57°C, no change in optical properties was observed. Results suggest that the decrease in fluorescence is both a property of fluorescence and a result of altering optical properties.
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Alex Walsh, Bart Masters, Duco Jansen, A. J. Welch, and Anita Mahadevan-Jansen "The effect of temperature of fluorescence: an animal study", Proc. SPIE 7562, Optical Interactions with Tissues and Cells XXI, 75620W (11 February 2010);

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