23 February 2010 Enhanced detection of circulating melanoma cells using gold nanoparticles as photoacoustic contrasting agents
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Abstract
Nanotechnology and the various properties of gold nanoparticles (AuNPs) are quickly changing the field of cancer detection and treatment. Photoacoustic detection methods show an increase in sensitivity using gold nanoparticle antibody conjugation, which selectively targets melanoma cancer cells. Instead of targeting melanoma tumors, we tag single cells, analogous to circulating metastatic melanoma cells. Using an in vitro, stationary cell system and planar samples, we demonstrate an average of 24% improved optical detectability of melanoma cells tagged with AuNPs over unprocessed melanoma cells. Tagged cells showed a raised plateau of absorbance from 470nm to 550nm. Untagged cells showed a general decline in absorption as wavelength increased. The results of our study have the potential to not only better develop photoacoustic detection of melanoma, but also extend the viability and use of photoacoustics into detection of otherwise unpigmented cancers.
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Devin R. McCormack, Devin R. McCormack, Kiran Bhattacharyya, Kiran Bhattacharyya, Raghuraman Kannan, Raghuraman Kannan, Kattesh Katti, Kattesh Katti, John A. Viator, John A. Viator, } "Enhanced detection of circulating melanoma cells using gold nanoparticles as photoacoustic contrasting agents", Proc. SPIE 7564, Photons Plus Ultrasound: Imaging and Sensing 2010, 756413 (23 February 2010); doi: 10.1117/12.841474; https://doi.org/10.1117/12.841474
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