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23 February 2010 Frequency-selective multiphoton-excitation-induced photoacoustic microscopy (MEPAM) to visualize the cross sections of dense objects
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Multiphoton excitation-induced photoacoustic microscopy (MEPAM) can be used to investigate the interior of dense objects precisely and directly because the multiphoton excitation occurs only at the focal point. This method makes it possible to avoid the strong signal from the surface of dense objects. However, in the case of tissue imaging, one-photon photoacoustic signals affect the image constructed from MEPAM signals, owing to the smaller cross section of multiphoton absorption compared with that of one-photon absorption. Thus, in order to apply MEPAM for precise investigation in living tissues, it is important to enhance (or extract) only the photoacoustic signals induced by multiphoton excitation. In this study, we examined the use of frequency-selective detection (frequency filtering) in multiphotonphotoacoustic imaging by evaluating the depth discrimination and penetration. Because MEPAM signals are generated in a very small region, they include higher frequency components compared with one-photon photoacoustic signals. We measured the images at the cross sections of blood-vessel phantoms visualized by MEPAM using the high-frequency components. We found that the images visualized using only high-frequency components showed better contrast compared with those visualized using all frequency components. We conclude that the combination of frequency filtering and MEPAM demonstrates great potential for precise observation of cross sections of blood vessels in living tissues.
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Yoshihisa Yamaoka, Mika Nambu, and Tetsuro Takamatsu "Frequency-selective multiphoton-excitation-induced photoacoustic microscopy (MEPAM) to visualize the cross sections of dense objects", Proc. SPIE 7564, Photons Plus Ultrasound: Imaging and Sensing 2010, 75642O (23 February 2010);

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