This paper reports a robust polymer based centrifugal microfluidic analysis system that can provide parallel
detection of multiple allergens in vitro. Many commercial food products (milk, bean, pollen, etc.) may introduce allergy
to people. A low-cost device for rapid detection of allergens is highly desirable. With this as the objective, we have
studied the feasibility of using a rotating disk device incorporating centrifugal microfluidics for performing actuationfree
and multi-analyte detection of different allergen species with minimum sample usage and fast response time.
Degranulation in basophils or mast cells is an indicator to demonstrate allergic reaction. In this connection, we used
acridine orange (AO) to demonstrate degranulation in KU812 human basophils. It was found that the AO was released
from granules when cells were stimulated by ionomycin, thus signifying the release of histamine which accounts for
allergy symptoms [1-2]. Within this rotating optical platform, major microfluidic components including sample reservoirs,
reaction chambers, microchannel and flow-control compartments are integrated into a single bio-compatible
polydimethylsiloxane (PDMS) substrate. The flow sequence and reaction time can be controlled precisely. Sequentially
through varying the spinning speed, the disk may perform a variety of steps on sample loading, reaction and detection.
Our work demonstrates the feasibility of using centrifugation as a possible immunoassay system in the future.