11 February 2010 A fluorescence-based centrifugal microfluidic system for parallel detection of multiple allergens
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Proceedings Volume 7565, Biophotonics and Immune Responses V; 75650F (2010) https://doi.org/10.1117/12.840914
Event: SPIE BiOS, 2010, San Francisco, California, United States
Abstract
This paper reports a robust polymer based centrifugal microfluidic analysis system that can provide parallel detection of multiple allergens in vitro. Many commercial food products (milk, bean, pollen, etc.) may introduce allergy to people. A low-cost device for rapid detection of allergens is highly desirable. With this as the objective, we have studied the feasibility of using a rotating disk device incorporating centrifugal microfluidics for performing actuationfree and multi-analyte detection of different allergen species with minimum sample usage and fast response time. Degranulation in basophils or mast cells is an indicator to demonstrate allergic reaction. In this connection, we used acridine orange (AO) to demonstrate degranulation in KU812 human basophils. It was found that the AO was released from granules when cells were stimulated by ionomycin, thus signifying the release of histamine which accounts for allergy symptoms [1-2]. Within this rotating optical platform, major microfluidic components including sample reservoirs, reaction chambers, microchannel and flow-control compartments are integrated into a single bio-compatible polydimethylsiloxane (PDMS) substrate. The flow sequence and reaction time can be controlled precisely. Sequentially through varying the spinning speed, the disk may perform a variety of steps on sample loading, reaction and detection. Our work demonstrates the feasibility of using centrifugation as a possible immunoassay system in the future.
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Q. L. Chen, H. P. Ho, K. L. Cheung, S. K. Kong, Y. K. Suen, Y. W. Kwan, W. J. Li, C. K. Wong, "A fluorescence-based centrifugal microfluidic system for parallel detection of multiple allergens", Proc. SPIE 7565, Biophotonics and Immune Responses V, 75650F (11 February 2010); doi: 10.1117/12.840914; https://doi.org/10.1117/12.840914
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