Involvement of ASK1 activation in apoptosis induced by NPe6-PDT

Lei Liu; Zhen-zhen Zhang; Zhigang Zhang

doi:10.1117/12.840472

11 February 2010 Involvement of ASK1 activation in apoptosis induced by NPe6-PDT

Lei Liu, Zhen-zhen Zhang, Zhigang Zhang

Proceedings Volume 7565, Biophotonics and Immune Responses V; 75650L (2010) https://doi.org/10.1117/12.840472
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Photodynamic therapy (PDT) employing photosensiter N-aspartyl chlorin e6 (NPe6) can induce lysosome disruption and initiate apoptotic pathway. Apoptosis signal-regulating kinase (ASK1) is an important regulator of apoptosis in response to various stresses, such as reactive oxygen species (ROS), endoplasmic reticulum (ER) stress, lipopolysaccharide (LPS) and calcium influx. In this study, we investigated the molecular mechanisms of apoptosis induced by NPe6-PDT in ASTC-a-1 cells. The results showed that the activities of ASK1 increased in response to NPe6-PDT. Over-expression of wild-type or activated mutant of ASK1 could obviously decrease cell viability and increase cell death; while inhibition of ASK1 significantly decreased cell apoptosis. These results suggested that ASK1 plays an important role in apoptosis induced by NPe6-PDT.

Citation Download Citation

Lei Liu, Zhen-zhen Zhang, and Zhigang Zhang "Involvement of ASK1 activation in apoptosis induced by NPe6-PDT", Proc. SPIE 7565, Biophotonics and Immune Responses V, 75650L (11 February 2010); https://doi.org/10.1117/12.840472

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