Redistribution of intramitochondrial cardiolipin at the early stage of apoptosis is associated with ROS

Zhiyong He; Da Xing; Lei Liu; Sihua Yang

doi:10.1117/12.840840

11 February 2010 Redistribution of intramitochondrial cardiolipin at the early stage of apoptosis is associated with ROS

Zhiyong He, Da Xing, Lei Liu, Sihua Yang

Author Affiliations +

Proceedings Volume 7565, Biophotonics and Immune Responses V; 75650N (2010) https://doi.org/10.1117/12.840840
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Cardiolipin is a unique and ubiquitous diphosphatidylglycerol phospholipid, located exclusively in inner membrane of mitochondria and particularly intermembrane contact sites. Cardiolipin is essential for mitochondrial to maintain its functions. Numerous mitochondrial proteins and processes require the presence of cardiolipin. Recent researches gradually confirm that cardiolipin participates in several mitochondria-dependent apoptotic steps: interactions between cardiolipin and cytochrome c, Bid and caspase-8 have now been established. These functions are associated with the redistribution of cardiolipin in mitochondria. However, the exact mechanism of the redistribution, which happens at the early stage of apoptosis, is still controversial. In this study, we used 10-N-nonyl-3, 6-bis (dimethylamino) acridine (10-N-nonyl acridine orange), a specific probe for cardiolipin to monitor changes of cardiolipin redistribution during apoptosis. We demonstrated that during apoptosis cardiolipin moves to the outer leaflet of mitochondrial inner membrane from the inner leaflet, where it used to be riched in. We also found that ROS (reactive oxygen species) may have association with the redistribution of cardiolipin.

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Zhiyong He, Da Xing, Lei Liu, and Sihua Yang "Redistribution of intramitochondrial cardiolipin at the early stage of apoptosis is associated with ROS", Proc. SPIE 7565, Biophotonics and Immune Responses V, 75650N (11 February 2010); https://doi.org/10.1117/12.840840

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