Two photon microscopy intravital study of DC-mediated anti-tumor response of NK cells

Michele Caccia; Tatiana Gorletta; Laura Sironi; Ivan Zanoni; Cristina Salvetti; Maddalena Collini; Francesca Granucci; Giuseppe Chirico

doi:10.1117/12.842214

11 February 2010 Two photon microscopy intravital study of DC-mediated anti-tumor response of NK cells

Michele Caccia, Tatiana Gorletta, Laura Sironi, Ivan Zanoni, Cristina Salvetti, Maddalena Collini, Francesca Granucci, Giuseppe Chirico

Author Affiliations +

Proceedings Volume 7565, Biophotonics and Immune Responses V; 75650Q (2010) https://doi.org/10.1117/12.842214
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Recent studies have demonstrated that dendritic cells (DCs) play a crucial role in the activation of Natural Killer cells (NKs) that are responsible for anti-tumor innate immune responses. The focus of this report is on the role of pathogen associated molecular pattern (PAMP) activated-DCs in inducing NK cell-mediated anti-tumor responses. Mice transplanted sub-cute (s.c.) with AK7 cells, a mesothelioma cell line sensitive to NK cell responses, are injected with fluorescent NK cells and DC activation is then induced by s.c. injection of Lipopolysaccharide (LPS). Using 4 dimensional tracking we follow the kinetic behavior of NK cells at the Draining Lymph-Node (DLN). As control, noninflammatory conditions are also evaluated. Our data suggest that NK cells are recruited to the DLN where they can interact with activated-DCs with a peculiar kinetic behavior: short lived interactions interleaved by rarer longer ones. We also found that the changes in the NK dynamic behavior in inflammatory conditions clearly affect relevant motility parameters such as the instantaneous and average velocity and the effective diffusion coefficient. This observation suggests that NK cells and activated-DCs might efficiently interact in the DLN, where cells could be activated. Therefore the interaction between activated-DCs and NK cells in DLN is not only a reality but it may be also crucial for the start of the immune response of the NKs.

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Michele Caccia, Tatiana Gorletta, Laura Sironi, Ivan Zanoni, Cristina Salvetti, Maddalena Collini, Francesca Granucci, and Giuseppe Chirico "Two photon microscopy intravital study of DC-mediated anti-tumor response of NK cells", Proc. SPIE 7565, Biophotonics and Immune Responses V, 75650Q (11 February 2010); https://doi.org/10.1117/12.842214

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