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24 February 2010Dynamic phase imaging of host cells attacked by Vibrio vulnificus using quantitative phase microscopy
We present the real time quantitative analysis of Vibriovulnificus-infected host cells using high stability quantitative
phase microscopy (HSQPM). It provides the ability to retrieve the phase or optical path length distribution over the cell
from a single interferogram image, which has been measured with nanometer path length sensitivity for long periods of
time. We have applied HSQPM to study dynamic cell morphologic changes and to quantify noninvasively cell volumes
of rat basophilic leukemia RBL-2H3 cells infected with pathogenic bacteria V. vulnificus strains, wild type (MO6-24/O)
and RTX toxin mutant (CMM770). During the process of V. vulnificus wild type infection to RBL-2H3 cells, the
dynamic changes of quantitative phase images, cell volumes and areas were observed in real time using HSQPM. In
contrast, the dramatic changes were not detected in RBL-2H3 cells infected with RTX toxin mutant. The results showed
the good correlation between HSQPM analysis and biochemical assays such as lactate dehydrogenase (LDH) assay and
β-hexosaminidase release assay. We suggest that HSQPM is useful real time quantitative method to study the dynamic
process of host cells infected with pathogen in a noninvasive manner.
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Seungrag Lee, Wenzhong Yang, Ji Yong Lee, Mi Hye Cha, Young Ran Kim, Dug Young Kim, "Dynamic phase imaging of host cells attacked by vibrio vulnificus using quantitative phase microscopy," Proc. SPIE 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII, 756808 (24 February 2010); https://doi.org/10.1117/12.843057