Spectral ophthalmoscopy based on supercontinuum

Yueh-Hung Cheng; Jiun-Yann Yu; Han-Hsuan Wu; Bo-Jyun Huang; Shi-Wei Chu

doi:10.1117/12.843020

24 February 2010 Spectral ophthalmoscopy based on supercontinuum

Yueh-Hung Cheng, Jiun-Yann Yu, Han-Hsuan Wu, Bo-Jyun Huang, Shi-Wei Chu

Proceedings Volume 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII; 75680I (2010) https://doi.org/10.1117/12.843020
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Confocal scanning laser ophthalmoscope (CSLO) has been established to be an important diagnostic tool for retinopathies like age-related macular degeneration, glaucoma and diabetes. Compared to a confocal laser scanning microscope, CSLO is also capable of providing optical sectioning on retina with the aid of a pinhole, but the microscope objective is replaced by the optics of eye. Since optical spectrum is the fingerprint of local chemical composition, it is attractive to incorporate spectral acquisition into CSLO. However, due to the limitation of laser bandwidth and chromatic/geometric aberration, the scanning systems in current CSLO are not compatible with spectral imaging. Here we demonstrate a spectral CSLO by combining a diffraction-limited broadband scanning system and a supercontinuum laser source. Both optical sectioning capability and sub-cellular resolution are demonstrated on zebrafish's retina. To our knowledge, it is also the first time that CSLO is applied onto the study of fish vision. The versatile spectral CSLO system will be useful to retinopathy diagnosis and neuroscience research.

Citation Download Citation

Yueh-Hung Cheng, Jiun-Yann Yu, Han-Hsuan Wu, Bo-Jyun Huang, and Shi-Wei Chu "Spectral ophthalmoscopy based on supercontinuum", Proc. SPIE 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII, 75680I (24 February 2010); https://doi.org/10.1117/12.843020

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