Comparing coherent and spontaneous Raman scattering signals for biological imaging applications

Brandon R. Bachler; Meng Cui; Sarah R. Nichols; Jennifer P. Ogilvie

doi:10.1117/12.842847

26 February 2010 Comparing coherent and spontaneous Raman scattering signals for biological imaging applications

Brandon R. Bachler, Meng Cui, Sarah R. Nichols, Jennifer P. Ogilvie

Author Affiliations +

Proceedings Volume 7569, Multiphoton Microscopy in the Biomedical Sciences X; 75690O (2010) https://doi.org/10.1117/12.842847
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

We present a systematic comparison between coherent and spontaneous Raman scattering under conditions relevant for biological imaging. Using spectral domain imaging, we find that the signal levels for each method are comparable at the low excitation power and low concentrations appropriate for biological samples. For samples of polystyrene beads with a molecular concentration of 10 M, we determine the critical power at which the two methods give equal signal levels to be ~1.3 mW. The advantages offered by coherent Raman methods are mitigated by the low excitation power, low sample concentrations, and short interaction lengths involved with biological imaging. We present calculations to support our measurements.

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Brandon R. Bachler, Meng Cui, Sarah R. Nichols, and Jennifer P. Ogilvie "Comparing coherent and spontaneous Raman scattering signals for biological imaging applications", Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 75690O (26 February 2010); https://doi.org/10.1117/12.842847

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