Assessment of fibrotic liver disease with multimodal nonlinear optical microscopy

Fake Lu; Wei Zheng; Dean C. S. Tai; Jian Lin; Hanry Yu; Zhiwei Huang

doi:10.1117/12.843127

26 February 2010 Assessment of fibrotic liver disease with multimodal nonlinear optical microscopy

Fake Lu, Wei Zheng, Dean C. S. Tai, Jian Lin, Hanry Yu, Zhiwei Huang

Proceedings Volume 7569, Multiphoton Microscopy in the Biomedical Sciences X; 75691W (2010) https://doi.org/10.1117/12.843127
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Liver fibrosis is the excessive accumulation of extracellular matrix proteins such as collagens, which may result in cirrhosis, liver failure, and portal hypertension. In this study, we apply a multimodal nonlinear optical microscopy platform developed to investigate the fibrotic liver diseases in rat models established by performing bile duct ligation (BDL) surgery. The three nonlinear microscopy imaging modalities are implemented on the same sectioned tissues of diseased model sequentially: i.e., second harmonic generation (SHG) imaging quantifies the contents of the collagens, the two-photon excitation fluorescence (TPEF) imaging reveals the morphology of hepatic cells, while coherent anti-Stokes Raman scattering (CARS) imaging maps the distributions of fats or lipids quantitatively across the tissue. Our imaging results show that during the development of liver fibrosis (collagens) in BDL model, fatty liver disease also occurs. The aggregated concentrations of collagen and fat constituents in liver fibrosis model show a certain correlationship between each other.

Citation Download Citation

Fake Lu, Wei Zheng, Dean C. S. Tai, Jian Lin, Hanry Yu, and Zhiwei Huang "Assessment of fibrotic liver disease with multimodal nonlinear optical microscopy", Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 75691W (26 February 2010); https://doi.org/10.1117/12.843127

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KEYWORDS

Liver

Collagen

Microscopy

Tissues

Optical microscopy

Second-harmonic generation

CARS tomography

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