17 March 2010 Multimodal nonlinear microscopy at 1.5 µm
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We describe multimodal nonlinear microscopy using a compact, turnkey femtosecond fiber laser at 1.5 μm. The system allows for multiplexed detection of near infrared and visible contrast agents through two-and three-photon excitation fluorescence microscopy as well as structural imaging viaauto-confocal microscopy (ACM). This platform expands the available emission spectrum for multiphoton microscopy, enables simultaneous structural and functional imaging, and offers advantages in penetration depth, contrast, and simplicity as compared to conventional MPM near 800 nm excitation.
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Chun Zhan, Chun Zhan, Chulmin Joo, Chulmin Joo, Qing Li, Qing Li, Mikhail Y. Berezin, Mikhail Y. Berezin, Walter J. Akers, Walter J. Akers, Yunpeng Ye, Yunpeng Ye, Samuel Achilefu, Samuel Achilefu, Siavash Yazdanfar, Siavash Yazdanfar, } "Multimodal nonlinear microscopy at 1.5 µm", Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 75691Z (17 March 2010); doi: 10.1117/12.842573; https://doi.org/10.1117/12.842573

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