Multimodal nonlinear microscopy at 1.5 µm

Chun Zhan; Chulmin Joo; Qing Li; Mikhail Y. Berezin; Walter J. Akers; Yunpeng Ye; Samuel Achilefu; Siavash Yazdanfar

doi:10.1117/12.842573

17 March 2010 Multimodal nonlinear microscopy at 1.5 µm

Chun Zhan, Chulmin Joo, Qing Li, Mikhail Y. Berezin, Walter J. Akers, Yunpeng Ye, Samuel Achilefu, Siavash Yazdanfar

Proceedings Volume 7569, Multiphoton Microscopy in the Biomedical Sciences X; 75691Z (2010) https://doi.org/10.1117/12.842573
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

We describe multimodal nonlinear microscopy using a compact, turnkey femtosecond fiber laser at 1.5 μm. The system allows for multiplexed detection of near infrared and visible contrast agents through two-and three-photon excitation fluorescence microscopy as well as structural imaging viaauto-confocal microscopy (ACM). This platform expands the available emission spectrum for multiphoton microscopy, enables simultaneous structural and functional imaging, and offers advantages in penetration depth, contrast, and simplicity as compared to conventional MPM near 800 nm excitation.

Citation Download Citation

Chun Zhan, Chulmin Joo, Qing Li, Mikhail Y. Berezin, Walter J. Akers, Yunpeng Ye, Samuel Achilefu, and Siavash Yazdanfar "Multimodal nonlinear microscopy at 1.5 µm", Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 75691Z (17 March 2010); https://doi.org/10.1117/12.842573

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