Spatio-temporal control in multiphoton fluorescence laser-scanning microscopy

Arijit Kumar De; Debjit Roy; Debabrata Goswami

doi:10.1117/12.838287

26 February 2010 Spatio-temporal control in multiphoton fluorescence laser-scanning microscopy

Arijit Kumar De, Debjit Roy, Debabrata Goswami

Proceedings Volume 7569, Multiphoton Microscopy in the Biomedical Sciences X; 756929 (2010) https://doi.org/10.1117/12.838287
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

The broad spectral window of an ultra-short laser pulse and the broad overlapping multiphoton absorption spectra of common fluorophores restrict selective excitation of one fluorophore in presence of others during multiphoton fluorescence microscopy. Also spatial resolution, limited by the fundamental diffraction limit, is governed by the beam profile. Here we show our recent work on selective fluorescence suppression using a femtosecond pulse-pair excitation which is equivalent to amplitude shaping using a pulse shaper. In addition, prospects of laser beam shaping in imaging are also briefly discussed.

Citation Download Citation

Arijit Kumar De, Debjit Roy, and Debabrata Goswami "Spatio-temporal control in multiphoton fluorescence laser-scanning microscopy", Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 756929 (26 February 2010); https://doi.org/10.1117/12.838287

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