Multispectral autofluorescence lifetime imaging of RPE cells using two-photon excitation

Lingling Zhao; Danni Chen; Jing Qi; Junle Qu

doi:10.1117/12.845952

26 February 2010 Multispectral autofluorescence lifetime imaging of RPE cells using two-photon excitation

Lingling Zhao, Danni Chen, Jing Qi, Junle Qu

Proceedings Volume 7569, Multiphoton Microscopy in the Biomedical Sciences X; 75692B (2010) https://doi.org/10.1117/12.845952
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

In this paper, we present our investigation on multispectral autofluorescence lifetime imaging of RPE cells using two-photon excitation. Morphological characters of RPE cells are obtained with high spatial resolution. Different autofluorescence lifetime parameters have been compared at different emission bands. Spatial distribution of dominant endogenous fluorophores in RPE cells, such as FAD, A2E and AGE etc have been obtained by the analysis of τm and a1/a2 ratio in the whole emission spectrum.

Citation Download Citation

Lingling Zhao, Danni Chen, Jing Qi, and Junle Qu "Multispectral autofluorescence lifetime imaging of RPE cells using two-photon excitation", Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 75692B (26 February 2010); https://doi.org/10.1117/12.845952

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