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26 February 2010Multispectral autofluorescence lifetime imaging of RPE cells using two-photon excitation
In this paper, we present our investigation on multispectral autofluorescence lifetime imaging of RPE cells using two-photon
excitation. Morphological characters of RPE cells are obtained with high spatial resolution. Different
autofluorescence lifetime parameters have been compared at different emission bands. Spatial distribution of dominant
endogenous fluorophores in RPE cells, such as FAD, A2E and AGE etc have been obtained by the analysis of τm and
a1/a2 ratio in the whole emission spectrum.