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26 February 2010 High-resolution imaging of a corneal incision by second- and third-harmonic generation microscopy
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Abstract
We demonstrate high spatial resolution imaging of a stromal cut in the ex-vivo pig cornea, using second- and third-harmonic generation microscopy. From these images, we see in detail how the cut affects the corneal layers. In the beginning of the cut, the anterior layers, in which the blade is passing through, are disorganized, which could explain the shadows observed on the images. In the stroma, the cut can be imaged by third harmonic microscopy, probably due to the χ3 contrast. Although the current results were obtained from the healthy ex-vivo cornea, it already allows one to understand the effects of the cut on the tissue characteristics (such as scattering).
© (2010) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
L. Jay, C. Dion, A. Brocas, K. Singh, J.-C. Kieffer, I. Brunette, and T. Ozaki "High-resolution imaging of a corneal incision by second- and third-harmonic generation microscopy", Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 75692T (26 February 2010); https://doi.org/10.1117/12.842078
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