Polarization second harmonic generation (PSHG) imaging of neurons: estimating the effective orientation of the SHG source in axons

Sotiris Psilodimitrakopoulos; Valerie Petegnief; Guadalupe Soria; Ivan Amat-Roldan; David Artigas; Anna M. Planas; Pablo Loza-Alvarez

doi:10.1117/12.841313

26 February 2010 Polarization second harmonic generation (PSHG) imaging of neurons: estimating the effective orientation of the SHG source in axons

Sotiris Psilodimitrakopoulos, Valerie Petegnief, Guadalupe Soria, Ivan Amat-Roldan, David Artigas, Anna M. Planas, Pablo Loza-Alvarez

Author Affiliations +

Proceedings Volume 7569, Multiphoton Microscopy in the Biomedical Sciences X; 75692W (2010) https://doi.org/10.1117/12.841313
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

We use high resolution polarization second harmonic generation (PSHG) imaging microscopy in cultured neurons, and we provide estimation on the effective orientation of the SHG source in neuronal processes in vitro. We performed pixel by pixel analysis and we found a picked distribution of angles with maximum frequency at θ_e = 34.54°, with Δθ_e=11.44°. This angle value is very close to the inclination of the tubulin heterodimmer with respect to the long axis of the microtubule.

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Sotiris Psilodimitrakopoulos, Valerie Petegnief, Guadalupe Soria, Ivan Amat-Roldan, David Artigas, Anna M. Planas, and Pablo Loza-Alvarez "Polarization second harmonic generation (PSHG) imaging of neurons: estimating the effective orientation of the SHG source in axons", Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 75692W (26 February 2010); https://doi.org/10.1117/12.841313

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