26 February 2010 Polarization second harmonic generation (PSHG) imaging of neurons: estimating the effective orientation of the SHG source in axons
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Abstract
We use high resolution polarization second harmonic generation (PSHG) imaging microscopy in cultured neurons, and we provide estimation on the effective orientation of the SHG source in neuronal processes in vitro. We performed pixel by pixel analysis and we found a picked distribution of angles with maximum frequency at θe = 34.54°, with Δθe=11.44°. This angle value is very close to the inclination of the tubulin heterodimmer with respect to the long axis of the microtubule.
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Sotiris Psilodimitrakopoulos, Sotiris Psilodimitrakopoulos, Valerie Petegnief, Valerie Petegnief, Guadalupe Soria, Guadalupe Soria, Ivan Amat-Roldan, Ivan Amat-Roldan, David Artigas, David Artigas, Anna M. Planas, Anna M. Planas, Pablo Loza-Alvarez, Pablo Loza-Alvarez, } "Polarization second harmonic generation (PSHG) imaging of neurons: estimating the effective orientation of the SHG source in axons", Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 75692W (26 February 2010); doi: 10.1117/12.841313; https://doi.org/10.1117/12.841313
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