26 February 2010 Annular aperture detection scheme in radially polarized coherent anti-Stokes Raman scattering (RP-CARS) microscopy for contrast improvement
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Proceedings Volume 7569, Multiphoton Microscopy in the Biomedical Sciences X; 756939 (2010); doi: 10.1117/12.842942
Event: SPIE BiOS, 2010, San Francisco, California, United States
Abstract
We report on a unique annular aperture detection scheme in radially polarized coherent anti-Stokes Raman scattering (RP-CARS) microscopy to effectively remove the solvent background for high contrast vibrational imaging. Our finite-difference time-domain (FDTD) calculations show that the far-field RP-CARS radiation from the scatterer with size comparable to the excitation wavelength is stronger than that from the solvent at large cone angles (around 45° to 150°). The annular detection provides about one order higher contrast for both forward and backward detected RP-CARS microscopy.
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Jian Lin, Fake Lu, Haifeng Wang, Wei Zheng, C. J. R. Sheppard, Zhiwei Huang, "Annular aperture detection scheme in radially polarized coherent anti-Stokes Raman scattering (RP-CARS) microscopy for contrast improvement", Proc. SPIE 7569, Multiphoton Microscopy in the Biomedical Sciences X, 756939 (26 February 2010); doi: 10.1117/12.842942; https://doi.org/10.1117/12.842942
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KEYWORDS
Microscopy

CARS tomography

Finite-difference time-domain method

Objectives

Medical research

Signal detection

3D image processing

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