Extended depth-of-field microscopy

Christopher Smith; Edward Botcherby; Martin Booth; Rimas Juskaitis; Tony Wilson

doi:10.1117/12.842160

24 February 2010 Extended depth-of-field microscopy

Christopher Smith, Edward Botcherby, Martin Booth, Rimas Juskaitis, Tony Wilson

Proceedings Volume 7570, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII; 75700S (2010) https://doi.org/10.1117/12.842160
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

In this paper, we present details of a scanning two-photon fluorescence microscope we have built with a nearisotropic scan rate. This means that the focal spot can be scanned at high speed along any direction in the specimen, without introducing systematic aberrations. We present experimental point spread function measurements for this system using an Olympus 1.4 NA 60X oil immersion lens that demonstrates an axial range of operation greater than 70 μm. We give details of a novel actuator device used to displace the focusing element and demonstrate axial scan responses up to 3.5 kHz. Finally, we present an application of this system in liquid crystal research to image the dynamic response of a nematic device during switching. Information about the director field at different levels in the device can be inferred from images acquired with a temporal resolution of 2.5 ms.

Citation Download Citation

Christopher Smith, Edward Botcherby, Martin Booth, Rimas Juskaitis, and Tony Wilson "Extended depth-of-field microscopy", Proc. SPIE 7570, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII, 75700S (24 February 2010); https://doi.org/10.1117/12.842160

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