25 February 2010 Metal nanoparticle fluorophore: a powerful fluorescence probe in single cell imaging
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Metal nanoparticle fluorophores have been developed using metal-enhanced fluorescence (MEF) principle. Compared with the conventional organic fluorophores, the metal fluorophores display the increasing brightness and shortening lifetime as well as the lengthening photostability and reducing photoblinking. Conjugated the metal fluorophores on the surfaces of cell lines, the cell images were recorded on a scanning confocal microscopy in the either emission intensity or lifetime. The emission spots by the conjugated metal fluorophores were isolated distinctly from the cell images because of their brighter signals and shorter lifetimes. Collected in the three-dimension, the total number of emission signals could be counted quantitatively and the distribution could be described on the cell surfaces. It was noticed that the emission intensity over the cell image was increased with an increase of the number of metal fluorophore on the cell surface and simultaneously the lifetime was altered. A quantitative regression curve was achieved between the amount of metal fluorophore on the cell surface and the emission intensity or lifetime over the entire cell image. Based on this regression curve, the target molecules on the cell surfaces could be quantified readily through the cell intensity and/or lifetime at the single cell level instead of the direct count to the emission spots. As novel molecule imaging agents, these metal fluorophores are being applied in the quantification and distribution of target molecule on the cell surface for the clinical diagnostic research.
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Jian Zhang, Jian Zhang, Yi Fu, Yi Fu, Richard Y. Zhao, Richard Y. Zhao, Joseph R. Lakowicz, Joseph R. Lakowicz, } "Metal nanoparticle fluorophore: a powerful fluorescence probe in single cell imaging", Proc. SPIE 7571, Single Molecule Spectroscopy and Imaging III, 75710N (25 February 2010); doi: 10.1117/12.842550; https://doi.org/10.1117/12.842550

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