Near-field optical fluorescence correlation spectroscopy

José Pérez; Michael Herrmann; Dietmar Gradl; Andreas Naber

doi:10.1117/12.842793

25 February 2010 Near-field optical fluorescence correlation spectroscopy

José Pérez, Michael Herrmann, Dietmar Gradl, Andreas Naber

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Proceedings Volume 7571, Single Molecule Spectroscopy and Imaging III; 757118 (2010) https://doi.org/10.1117/12.842793
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

The application of Fluorescence Correlation Spectroscopy (FCS) to Near-field Scanning Optical Microscopy on diffusing molecules in solution is presented here. The ultra small excitation volume in such a technique allows measurements with fluorophore concentrations of up to 10μM, making FCS in biological environment at nearly native conditions possible.

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José Pérez, Michael Herrmann, Dietmar Gradl, and Andreas Naber "Near-field optical fluorescence correlation spectroscopy", Proc. SPIE 7571, Single Molecule Spectroscopy and Imaging III, 757118 (25 February 2010); https://doi.org/10.1117/12.842793

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