26 February 2010 Tumor specific lung cancer diagnostics with multiplexed FRET immunoassays
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Abstract
An optical multiplexed homogeneous (liquid phase) immunoassay based on FRET from a terbium complex to eight different fluorescent dyes is presented. We achieved highly sensitive parallel detection of four different lung cancer specific tumor markers (CEA, NSE, SCC and CYFRA21-1) within a single assay and show a proof-of-principle for 5- fold multiplexing. The method is well suited for fast and low-cost miniaturized point-of-care testing as well as for highthroughput screening in a broad range of in-vitro diagnostic applications.
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D. Geißler, D. Geißler, D. Hill, D. Hill, H.-G. Löhmannsröben, H.-G. Löhmannsröben, E. Thomas, E. Thomas, A. Lavigne, A. Lavigne, B. Darbouret, B. Darbouret, E. Bois, E. Bois, L. J. Charbonnière, L. J. Charbonnière, R. F. Ziessel, R. F. Ziessel, N. Hildebrandt, N. Hildebrandt, } "Tumor specific lung cancer diagnostics with multiplexed FRET immunoassays", Proc. SPIE 7572, Optical Diagnostics and Sensing X: Toward Point-of-Care Diagnostics, 75720C (26 February 2010); doi: 10.1117/12.842731; https://doi.org/10.1117/12.842731
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