Limit of detection for a bead-based diffraction biosensor

Youbong Lim; David D. Nolte; Khalid Arif; Cagri Savran

doi:10.1117/12.842507

12 February 2010 Limit of detection for a bead-based diffraction biosensor

Youbong Lim, David D. Nolte, Khalid Arif, Cagri Savran

Proceedings Volume 7574, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VII; 75740W (2010) https://doi.org/10.1117/12.842507
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Diffraction-based biosensors that rely on optical scattering are a sensitive approach for biomolecular detection. We present a Mie surface double-interaction computer study of the in situ assembly of bead-based diffraction gratings on a gold substrate. The limit of detection for a system having 26 stripes is calculated to be approximately 25 beads in total, or approximately one bead per stripe in an immunoassay experiment. This sensitivity limit is orders of magnitude better than label-free molecular sensors, and is consistent with high-speed scanning for high-throughput assays.

Citation Download Citation

Youbong Lim, David D. Nolte, Khalid Arif, and Cagri Savran "Limit of detection for a bead-based diffraction biosensor", Proc. SPIE 7574, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VII, 75740W (12 February 2010); https://doi.org/10.1117/12.842507

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