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17 February 2010 Quantum dot-fluorescent protein pair as ratiometric pH sensor
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Abstract
Fluorescence resonance energy transfer (FRET) between a quantum dot (QD) and the pH-sensitive fluorescent protein mOrange has been used to develop a fluorescent pH-indicator that is bright and photostable enough for applications in fluorescence imaging, including the tracking of molecules through endocytic pathways. As the molar extinction coefficient of mOrange increases with pH, the ratio of the mOrange emission to the QD emission (FA/FD) increases sharply, producing greater than 10-fold increases in the FA/FD ratio between pH 4.5 and 7.5. This probe has been thoroughly characterized and it intracellular imaging potential explored.
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Allison M. Dennis and Gang Bao "Quantum dot-fluorescent protein pair as ratiometric pH sensor", Proc. SPIE 7575, Colloidal Quantum Dots for Biomedical Applications V, 75750C (17 February 2010); https://doi.org/10.1117/12.842640
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