17 February 2010 Optical size determination of quantum dots using FRET with terbium complexes as donors
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We present the use of luminescent terbium complexes (LTCs) as FRET donors and luminescent semiconductor quantum dots (QDs) as FRET acceptors for spectroscopic ruler measurements. The LTCs were labeled to polyhistidine-appended peptides which self-assembled onto three different QDs allowing optical multiplexed measurements. Forster distances were in the range of 60-75 Å and FRET efficiencies of up to 97 % were realized. Time-resolved analysis allowed the determination of donor-acceptor separation distances. The results suggests the efficient use of our LTC-to-QD FRET systems for multiplexed optical size determination, molecular ruler measurements and multi-parameter diagnostics.
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D. Geißler, D. Geißler, H.-G. Löhmannsröben, H.-G. Löhmannsröben, L. J. Charbonnière, L. J. Charbonnière, R. F. Ziessel, R. F. Ziessel, N. G. Butlin, N. G. Butlin, I. L. Medintz, I. L. Medintz, H. Mattoussi, H. Mattoussi, N. Hildebrandt, N. Hildebrandt, } "Optical size determination of quantum dots using FRET with terbium complexes as donors", Proc. SPIE 7575, Colloidal Quantum Dots for Biomedical Applications V, 75750E (17 February 2010); doi: 10.1117/12.842750; https://doi.org/10.1117/12.842750

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