17 February 2010 Biocompatible water soluble quantum dots as new biophotonic tools for hematologic cells: applications for flow cell cytometry
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Abstract
Quantum dots (QDs) are a promising class of fluorescent probes that can be conjugated to a variety of specific cell antibodies. For this reason, simple, cheap and reproducible routes of QDs´s syntheses are the main goal of many researches in this field. The main objective of this work was to demonstrate the ability of QDs as biolabels for flow cell cytometry analysis. We have synthesized biocompatible water soluble CdS/Cd(OH)2 and CdTe/CdS QDs and applied them as fluorescent labels of hematologic cells. CdTe/CdS QDs was prepared using using a simple aqueous route with mercaptoacetic acid and mercaptopropionic acid as stabilizing agents. The resulting CdTe/CdS QDs can target biological membrane proteins and can also be internalized by cells. We applied the CdTe/CdS QDs as biolabels of human lymphocytes and compared the results obtained for lymphocytes treated and non-treated with permeabilizing agents for cell membranes. Permeabilized cells present higher fluorescence pattern than non permeabilized ones. We associated antibody A to the CdS/Cd(OH)2 QDs to label type A red blood cell (RBC). In this case, the O erythrocytes were used as the negative control. The results demonstrate that QDs were successfully functionalized with antibody A. There was a specific binding of QDs-antibody A to RBC membrane antigen only for A RBCs. We have also monitored QDs-hematologic cell interaction by using fluorescence microscopy. Our method shows that QDs can be conjugated to a variety of specific cell antibodies and can become a potential, highly efficient and low cost diagnostic tool for flow cell cytometry, very compatible with the lasers and filters used in this kind of equipments.
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Rafael B. Lira, Antonio T. de Sales Neto, Kilmara K. H. G. Carvalho, Elisa S. Leite, Aluizio G. Brasil, Denise P. L. Azevedo, Paulo E. Cabral Filho, Mariana B. Cavalcanti, Ademir J. Amaral, Patricía M. A. Farias, Beate S. Santos, Adriana Fontes, "Biocompatible water soluble quantum dots as new biophotonic tools for hematologic cells: applications for flow cell cytometry", Proc. SPIE 7575, Colloidal Quantum Dots for Biomedical Applications V, 75750P (17 February 2010); doi: 10.1117/12.842660; https://doi.org/10.1117/12.842660
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