17 February 2010 Design and fabrication of a PDMS microchip based immunoassay
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In this paper, we describe the design and fabrication of a polydimethylsiloxane (PDMS) microchip for on-chip multiplex immunoassay applications. The microchip consists of a PDMS microfluidic channel layer and a micro pneumatic valve control layer. By selectively pressurizing the pneumatic microvalves, immuno reagents were controlled to flow and react in certain fluidic channel sites. Cross contamination was prevented by tightly closed valves. Our design was proposed to utilize PDMS micro channel surface as the solid phase immunoassay substrate and simultaneously detect four targets antigens on chip. Experimental results show that 20psi valve pressure is sufficient to tightly close a 200μm wide micro channel with flow rate up to 20μl/min.
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Guocheng Shao, Guocheng Shao, Wanjun Wang, Wanjun Wang, Jun Wang, Jun Wang, Yuehe Lin, Yuehe Lin, "Design and fabrication of a PDMS microchip based immunoassay", Proc. SPIE 7593, Microfluidics, BioMEMS, and Medical Microsystems VIII, 75930Q (17 February 2010); doi: 10.1117/12.847138; https://doi.org/10.1117/12.847138

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