Flow visualization and flow cytometry with holographic video microscopy

Fook Chiong Cheong; Bo Sun; Remi Dreyfus; Jesse Amato-Grill; Ke Xiao; Lisa Dixon; David G. Grier

doi:10.1117/12.840550

10 February 2010 Flow visualization and flow cytometry with holographic video microscopy

Fook Chiong Cheong, Bo Sun, Remi Dreyfus, Jesse Amato-Grill, Ke Xiao, Lisa Dixon, David G. Grier

Proceedings Volume 7619, Practical Holography XXIV: Materials and Applications; 761905 (2010) https://doi.org/10.1117/12.840550
Event: SPIE OPTO, 2010, San Francisco, California, United States

Abstract

The video stream captured by an in-line holographic microscope can be analyzed on a frame-by-frame basis to track individual colloidal particles' three-dimensional motions with nanometer resolution, and simultaneously to measure their sizes and refractive indexes. An efficient particle-tracking algorithm automates initial position estimation with sufficient accuracy to enable unattended holographic particle tracking and characterization. In this work, we demonstrated this approach to flow visualization in a microfluidic channel and also to flow cytometry of micrometer-scale colloidal spheres.

Citation Download Citation

Fook Chiong Cheong, Bo Sun, Remi Dreyfus, Jesse Amato-Grill, Ke Xiao, Lisa Dixon, and David G. Grier "Flow visualization and flow cytometry with holographic video microscopy", Proc. SPIE 7619, Practical Holography XXIV: Materials and Applications, 761905 (10 February 2010); https://doi.org/10.1117/12.840550

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