This work optimized a multi-pinhole collimator for a stationary three-camera Single Photon Emission Computed
Tomography (SPECT) system designed for rapid (one-second) dynamic imaging through simulations. Multi-pinhole
collimator designs were investigated to increase efficiency and angular sampling while maintaining adequate spatial
resolution for small-animal imaging. The study first analytically investigated the tradeoffs between efficiency and
spatial resolution as a function of the number of pinholes. An oval arrangement of pinholes was proposed, and the
benefits compared to a circular arrangement were quantified through simulations. Finally, collimators with six to nine
pinholes were simulated, and the resulting data compared with respect to efficiency, signal-to-noise ratio (SNR), and the
angular coverage in Radon space. All simulations used the GATE Monte Carlo toolkit. The results suggest that an oval arrangement of nine pinholes improved the efficiency and SNR by factors of 1.65 and 1.3, respectively, compared to a circular arrangement. A nine-pinhole collimator was found to provide the highest geometric efficiency (~6.35*104cps/mCi) and improved the SNR by a factor of ~1.3 and ~1.1 compared to the six- and eight-pinhole collimators, respectively. Overall, the simulated multi-pinhole system depicted cylindrical objects despite the limited angular sampling and scan time of the one-second, stationary three-camera acquisition.