9 March 2010 Quantification of fluorescent spots in time series of 3D confocal microscopy images of endoplasmic reticulum exit sites based on the HMAX transform
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Abstract
We present an approach for the quantification of fluorescent spots in time series of 3-D confocal microscopy images of endoplasmic reticulum exit sites of dividing cells. Fluorescent spots are detected based on extracted image regions of highest response using the HMAX transform and prior convolution of the 3-D images with a Gaussian kernel. The sensitivity of the involved parameters was studied and a quantitative evaluation using both 3-D synthetic and 3-D real data was performed. The approach was successfully applied to more than one thousand 3-D confocal microscopy images.
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Petr Matula, Petr Matula, Fatima Verissimo, Fatima Verissimo, Stefan Wörz, Stefan Wörz, Roland Eils, Roland Eils, Rainer Pepperkok, Rainer Pepperkok, Karl Rohr, Karl Rohr, } "Quantification of fluorescent spots in time series of 3D confocal microscopy images of endoplasmic reticulum exit sites based on the HMAX transform", Proc. SPIE 7626, Medical Imaging 2010: Biomedical Applications in Molecular, Structural, and Functional Imaging, 76261H (9 March 2010); doi: 10.1117/12.844441; https://doi.org/10.1117/12.844441
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