9 March 2010 Hardware and software system for automatic microemulsion assay evaluation by analysis of optical properties
Author Affiliations +
Abstract
A new hardware device called Microemulsion Analyzer (MEA), which facilitates the preparation and evaluation of microemulsions, was developed. Microemulsions, consisting of three phases (oil, surfactant and water) and prepared on deep well plates according to the PDMPD method can be automatically evaluated by means of the optical properties. The ratio of ingredients to form a microemulsion strongly depends on the properties and the amounts of the used ingredients. A microemulsion assay is set up on deep well plates to determine these ratios. The optical properties of the ingredients change from turbid to transparent as soon as a microemulsion is formed. The MEA contains a frame and an imageprocessing and analysis algorithm. The frame itself consists of aluminum, an electro luminescent foil (ELF) and a camera. As the frame keeps the well plate at the correct position and angle, the ELF provides constant illumination of the plate from below. The camera provides an image that is processed by the algorithm to automatically evaluate the turbidity in the wells. Using the determined parameters, a phase diagram is created that visualizes the information. This build-up can be used to analyze microemulsion assays and to get results in a standardized way. In addition, it is possible to perform stability tests of the assay by creating special differential stability diagrams after a period of time.
© (2010) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Ulf Maeder, Ulf Maeder, Thomas Schmidts, Thomas Schmidts, Jan-Michael Burg, Jan-Michael Burg, Johannes T. Heverhagen, Johannes T. Heverhagen, Frank Runkel, Frank Runkel, Martin Fiebich, Martin Fiebich, "Hardware and software system for automatic microemulsion assay evaluation by analysis of optical properties", Proc. SPIE 7626, Medical Imaging 2010: Biomedical Applications in Molecular, Structural, and Functional Imaging, 762625 (9 March 2010); doi: 10.1117/12.845489; https://doi.org/10.1117/12.845489
PROCEEDINGS
9 PAGES


SHARE
Back to Top