Translator Disclaimer
8 September 2010 High sensitivity transition-tuned long period grating for label-free immunosensing
Author Affiliations +
Proceedings Volume 7653, Fourth European Workshop on Optical Fibre Sensors; 76531X (2010) https://doi.org/10.1117/12.866265
Event: (EWOFS'10) Fourth European Workshop on Optical Fibre Sensors, 2010, Porto, Portugal
Abstract
We present real-time monitoring of multiple biomolecular interactions by means of high refractive index (HRI) coated long period grating (LPG) working in transition region. The coating was deposited by dip-coating as a thin layer of few hundreds nanometers of atactic polystyrene (PS). The HRI coating was used to enhance the LPG sensitivity to refractive index changes of the surrounding medium and its thickness was determined in order to tune the coated device at the desired working point. To allow immobilization of specific biomolecules, the PS overlay surface was functionalized through bovine serum albumin (BSA) and glutaraldehyde. In particular, BSA was directly adsorbed on PS through strong hydrophobic interactions, then glutaraldehyde was linked atop exploiting the lysines side chains. This intermediate layer allowed to covalently immobilize human IgG (antibody) on the device surface. To prove the immobilization method, the interaction between IgG and its proper anti-human IgG (antigen) was studied. The four binding interactions were on-line monitored following the shift of the LPG attenuation band. The experiment also suggests a novel and interesting biofunctionalization approach of unreactive synthetic polymers with applications in immunosensing.
© (2010) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
P. Pilla, V. Malachovská, A. Sandomenico, M. Ruvo, M. Giordano, A. Cutolo, and A. Cusano "High sensitivity transition-tuned long period grating for label-free immunosensing", Proc. SPIE 7653, Fourth European Workshop on Optical Fibre Sensors, 76531X (8 September 2010); https://doi.org/10.1117/12.866265
PROCEEDINGS
4 PAGES


SHARE
Advertisement
Advertisement
Back to Top