24 April 2010 Preparation and properties of new complex sensing film for fiber optic glucose sensor
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Abstract
In this paper, a new complex sensing film containing both optical indicator and enzyme was prepared and its sensing properties were studied, using cellulose acetate (CA) as the carrier and tris (2,2'-blpyridyl) dichloro-ruthenium (II) hexahydrate (Ru(bpy)3Cl2) as the indicator. The cross-linking method was used to immobilize glucose oxidase (GOD). The immobilization conditions were optimized: the concentration of sodium periodate as 0.2 M and the reaction time as 30 min, those for ethanediamine as 0.03 M and 2.5 hours, those for GA as 1.5% (v/v) and 2 hours, those for GOD as 35 mg/ml and 21h. The optimal temperature and pH value for the catalytic properties of the sensing film are 38 °C and 7.0, respectively. A fiber optic glucose sensor with this complex sensing film has been studied. The results show that its detecting range is 100-600 mg/dl and its response time is less than 20 seconds.
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Jun Huang, Jun Huang, Chao Wang, Chao Wang, Yinquan Yuan, Yinquan Yuan, Hai Wang, Hai Wang, Liyun Ding, Liyun Ding, Dian Fan, Dian Fan, } "Preparation and properties of new complex sensing film for fiber optic glucose sensor", Proc. SPIE 7673, Advanced Environmental, Chemical, and Biological Sensing Technologies VII, 76730X (24 April 2010); doi: 10.1117/12.849379; https://doi.org/10.1117/12.849379
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