23 April 2010 Novel probes for visualizing reactive oxygen species in lipid membranes
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Abstract
This work describes the rationale behind the preparation of fluorescent probes for imaging lipid peroxyl radicals within membranes of living cells (fluorescent lipophilic antioxidants). The new probes are based on BODIPY dyes tethered to phenol moieties. We discuss the spectroscopic properties of these novel probes, specifically the BODIPY-α-tocopherol analogue B-TOH, and present a molecular level explanation, based on photoinduced electron transfer, that accounts for the significant emission enhancement that the probe BTOH experiences upon reaction with peroxyl free radicals. In addition to the spectroscopy results in homogeneous media, we also describe studies performed in model lipid membranes which show that the sensitivity of BTOH towards lipid peroxyl radicals is somewhat reduced when the probe is membrane embedded. Solutions to increase the sensitivity of the free radical probes are discussed based on the redox potential of BODIPY dyes.
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Katerina Krumova, Katerina Krumova, Gonzalo Cosa, Gonzalo Cosa, } "Novel probes for visualizing reactive oxygen species in lipid membranes", Proc. SPIE 7674, Smart Biomedical and Physiological Sensor Technologies VII, 767406 (23 April 2010); doi: 10.1117/12.850978; https://doi.org/10.1117/12.850978
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