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23 April 2010 A difference imaging technique for monitoring real-time changes in morphology within the cell, tissue, and organism spatial domain
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Abstract
Image subtraction has been an extremely useful tool for capturing subtle changes in pixel intensity with extremely high temporal resolution, and has been used for decades in the astronomy and metal corrosion fields. However, to date, image subtraction has not been used as a mainstream technique for investigating morphological changes in cells, tissues, or whole organisms. We introduce a user-friendly differential imaging technique for monitoring real time (~msec) changes in morphology within the micrometer to millimeter spatial scale. The technique is demonstrated by measuring morphological changes morphology for biomedical (bone stress), agricultural (crop root elongation), and environmental (zooplankton ecotoxicology) applications. Subtle changes in growth that would typically only be observed by highly skilled experts are easily resolved via image subtraction and the use of convolution kernels. When coupled with techniques characterizing real time biochemical transport (e.g., respiration, ion/substrate transport), physiology can be directly quantified with a high temporal and spatial resolution. Because of the ease of use, this technique can be readily applied to any field of science concerned with bridging the gap between form and function.
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E. S. McLamore, M. Stensberg, G. Yale, H. Ochoa-Acuna, M. Sepulveda, X. Sun, O. Akkus, and D. M. Porterfield "A difference imaging technique for monitoring real-time changes in morphology within the cell, tissue, and organism spatial domain", Proc. SPIE 7674, Smart Biomedical and Physiological Sensor Technologies VII, 76740F (23 April 2010); https://doi.org/10.1117/12.851694
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