20 April 2010 Micro-fabricated wireless biosensors for the detection of S. typhimurium in liquids
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Abstract
Food borne illnesses from the ingestion of S. typhimurium have been of primary concern due to their common occurrence in food products of daily consumption. In this paper, micron size, magnetoelastic (ME) biosensors for the detection of S. typhimurium were fabricated and tested in liquid solutions containing known concentrations of S. typhimurium cells. The biosensors are comprised of a ME sensor platform and immobilized bio-molecular recognition element (E2 phage) that has been engineered to bind the S. typhimurium multi-valently. The micron size ME sensor platforms were manufactured using microelectronics fabrication techniques. Phage was engineered at Auburn University and immobilized onto all surfaces of the sensor. The ME biosensor oscillates with a characteristic resonance frequency when subjected to a time varying magnetic field. Binding between the phage and bacteria, adds mass to the sensor that causes a shift in the sensor's resonance frequency. Sensors with the dimension of 500x100x4 μm were exposed to S. typhimurium with increasing known concentrations ranging from 5 x101 to 5 x 107 cfu/ml. The ME biosensor exhibited high sensitivity and a detection limit better than 50 cfu/ml.
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Suiqiong Li, Suiqiong Li, Michael L. Johnson, Michael L. Johnson, Yugui Li, Yugui Li, Huiqin Chen, Huiqin Chen, Ishita Banerjee, Ishita Banerjee, I-Hsuan Chen, I-Hsuan Chen, James M. Barbaree, James M. Barbaree, Z.-Y. Cheng, Z.-Y. Cheng, Bryan A. Chin, Bryan A. Chin, } "Micro-fabricated wireless biosensors for the detection of S. typhimurium in liquids", Proc. SPIE 7676, Sensing for Agriculture and Food Quality and Safety II, 76760N (20 April 2010); doi: 10.1117/12.849474; https://doi.org/10.1117/12.849474
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