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28 April 2010 Monitoring intra-cellular lipid metabolism in macrophages by Raman- and CARS-microscopy
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Abstract
Monocyte-derived macrophages play a key role in lipid metabolism in vessel wall tissues. Macrophages can take up lipids by various mechanisms. As phagocytes, macrophages are important for the decomposition of lipid plaques within arterial walls that contribute to arteriosclerosis. Of special interest are uptake dynamics and intra-cellular fate of different individual types of lipids as, for example, fatty acids, triglycerides or free and esterified cholesterol. Here we utilize Raman microscopy to image the metabolism of such lipids and follow subsequent storage or degradation patterns. The combination of optical microscopy with Raman spectroscopy allows visualization at the diffraction limit of the employed laser light and biochemical characterization through the associated spectral information. Relatively long measuring times, due to the weakness of Raman scattering can be overcome by non-linear effects such as coherent anti-Stokes Raman scattering (CARS). With this contribution we introduce first results to monitor the incorporation of lipid components into individual cells employing Raman and CARS microscopy.
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Christian Matthäus, Gero Bergner, Christoph Krafft, Benjamin Dietzek, Stefan Lorkowski, and Jürgen Popp "Monitoring intra-cellular lipid metabolism in macrophages by Raman- and CARS-microscopy", Proc. SPIE 7715, Biophotonics: Photonic Solutions for Better Health Care II, 771511 (28 April 2010); https://doi.org/10.1117/12.854244
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