17 May 2010 Biomolecular detection using a metal semiconductor field effect transistor
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In this work, our attention was drawn towards developing affinity-based electrical biosensors, using a MESFET (Metal Semiconductor Field Effect Transistor). Semiconductor (SC) surfaces must be prepared before the incubations with biomolecules. The peptides route was adapted to exceed and bypass the limits revealed by other types of surface modification due to the unwanted unspecific interactions. As these peptides reveal specific recognition of materials, then controlled functionalization can be achieved. Peptides were produced by phage display technology using a library of M13 bacteriophage. After several rounds of bio-panning, the phages presenting affinities for GaAs SC were isolated; the DNA of these specific phages were sequenced, and the peptide with the highest affinity was synthesized and biotinylated. To explore the possibility of electrical detection, the MESFET fabricated with the GaAs SC were used to detect the streptavidin via the biotinylated peptide in the presence of the bovine Serum Albumin. After each surface modification step, the IDS (current between the drain and the source) of the transistor was measured and a decrease in the intensity was detected. Furthermore, fluorescent microscopy was used in order to prove the specificity of this peptide and the specific localisation of biomolecules. In conclusion, the feasibility of producing an electrical biosensor using a MESFET has been demonstrated. Controlled placement, specific localization and detection of biomolecules on a MESFET transistor were achieved without covering the drain and the source. This method of functionalization and detection can be of great utility for biosensing application opening a new way for developing bioFETs (Biomolecular Field-Effect Transistor).
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Elias Estephan, Elias Estephan, Marie-Belle Saab, Marie-Belle Saab, Petre Buzatu, Petre Buzatu, Roger Aulombard, Roger Aulombard, Frédéric J. G. Cuisinier, Frédéric J. G. Cuisinier, Csilla Gergely, Csilla Gergely, Thierry Cloitre, Thierry Cloitre, } "Biomolecular detection using a metal semiconductor field effect transistor", Proc. SPIE 7715, Biophotonics: Photonic Solutions for Better Health Care II, 77151V (17 May 2010); doi: 10.1117/12.853536; https://doi.org/10.1117/12.853536

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