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18 May 2010 FT-IR microspectroscopy characterization of supports for enzyme immobilization in biosensing applications
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Abstract
The investigation of materials suitable for enzyme immobilization in biosensing applications has a widespread interest. There are many studies on physico-chemical properties of these materials at macroscopic level but few studies have been devoted to examine and correlate these properties at microscopic level. FT-IR spectroscopy with Micro-Attenuated Total Reflection (Micro-ATR) approach can be extremely useful for understanding a variety of aspects of materials which can be used for optimising immobilization procedures. Moreover, this experimental approach is particularly simple to use (no sample preparation is required) and minimally invasive. Using a Perkin Elmer Spectrum One FT-IR spectrometer equipped with a mercury-cadmium-telluride detector and a micro-ATR element we investigated different materials used for immobilization procedures with various enzymes widely used for biosensing in environmental and clinical applications. In particular, composite membranes constituted by a chemically modified poly-acrylonitrile (PAN) membrane plus layers of tethered chitosan of different molecular weight have been examined. Also silica gel matrices without and with glucose oxidase have been investigated. Spectra have been analysed and the contribution of principal functional groups has been evidenced.
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M. Portaccio, B. Della Ventura, K. Gabrovska, I. Marinov, T. Godjevargova, D. G. Mita, and M. Lepore "FT-IR microspectroscopy characterization of supports for enzyme immobilization in biosensing applications", Proc. SPIE 7715, Biophotonics: Photonic Solutions for Better Health Care II, 77153P (18 May 2010); https://doi.org/10.1117/12.852804
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