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A novel and sensitive electrochemical DNA biosensor based on multi-walled carbon nanotubes functionalized with a thio group (MWNTs-SH) and gold nanoparticles (GNPs) for covalent DNA immobilization and enhanced hybridization detection is described. The key step for developing this novel DNA biosensor is to cut the pristine MWNT into short and generate lots of active sites simultaneously. With this approach, the target DNA could be quantified in a linear range
from 8.5×10-10 to 1.5×10-5 mol/L, with a detection limit of 1.67×10-11 mol/L by 3σ.
Feng Guo,Jimei Zhang,Zhao Dai, andGuo Zheng
"Short thio-multi-walled carbon nanotubes and Au nanoparticles enhanced electrochemical DNA biosensor for DNA hybridization detection", Proc. SPIE 7749, 2010 International Conference on Display and Photonics, 77490H (21 July 2010); https://doi.org/10.1117/12.869660
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Feng Guo, Jimei Zhang, Zhao Dai, Guo Zheng, "Short thio-multi-walled carbon nanotubes and Au nanoparticles enhanced electrochemical DNA biosensor for DNA hybridization detection," Proc. SPIE 7749, 2010 International Conference on Display and Photonics, 77490H (21 July 2010); https://doi.org/10.1117/12.869660