Toward a hybridization assay using fluorescence resonance energy transfer and quantum dots immobilized in microfluidic channels

Anthony J. Tavares; Eleonora Petryayeva; W. Russ Algar; Lu Chen; Ulrich J. Krull

doi:10.1117/12.876746

14 September 2010 Toward a hybridization assay using fluorescence resonance energy transfer and quantum dots immobilized in microfluidic channels

Anthony J. Tavares, Eleonora Petryayeva, W. Russ Algar, Lu Chen, Ulrich J. Krull

Author Affiliations +

Proceedings Volume 7750, Photonics North 2010; 775003 (2010) https://doi.org/10.1117/12.876746
Event: Photonics North 2010, 2010, Niagara Falls, Canada

Abstract

Quantum dots (QDs) have been widely adopted as integrated components of bioassays and biosensors. In particular, solid phase nucleic acid hybridization assays have been demonstrated to have several advantages and permit the detection of up to four DNA targets simultaneously using fluorescence resonance energy transfer (FRET). This work explores the potential for miniaturization of a solid-phase nucleic acid hybridization assay using QDs and FRET on a microfluidics platform. A method was developed for the immobilization of Streptavidin coated QDs and the preparation of QD-probe oligonucleotide conjugates within microfluidic channels using electrokinetic delivery. Proof-of-concept was demonstrated for the selective detection of target DNA using FRET-sensitized emission from a Cy3 acceptor paired with a green emitting QD donor. The microfluidic platform offered the advantages of smaller sample volumes, nearly undetectable non-specific adsorption, and hybridization within minutes. This work is an important first step toward the development of biochips that enable the multiplexed detection of nucleic acid targets.

Citation Download Citation

Anthony J. Tavares, Anthony J. Tavares, Eleonora Petryayeva, Eleonora Petryayeva, W. Russ Algar, W. Russ Algar, Lu Chen, Lu Chen, Ulrich J. Krull, Ulrich J. Krull, "Toward a hybridization assay using fluorescence resonance energy transfer and quantum dots immobilized in microfluidic channels", Proc. SPIE 7750, Photonics North 2010, 775003 (14 September 2010); doi: 10.1117/12.876746; https://doi.org/10.1117/12.876746

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